Neutralization:
Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery
Article Snippet: .. For assessment of viral immune neutralization in vivo, AAV9-Cre (#105537, Addgene), Bald-LV-Cre, or Mymk+Mymg-LV-Cre were injected into the left TA muscle of (4–5)-week old Mdx 4cv ; Rosa26 tdTomato mice and 28 days later the same viral species were injected into the right TA of the same cohort of mice and muscle were harvested after 28 days and processed for histological analysis. . Immunoblotting Cell, viral, or extracellular vesicle pellets were lysed in NP-40 lysis buffer (50 mM Tris-Cl (pH 8.0), 200 mM NaCl, 50 mM NaF, 1 mM dithiotheritol, 1 mM sodium orthovanadate, 1 mM Benzamidine, 0.3% NP-40 and protease inhibitors). ..
In Vivo:
Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery
Article Snippet: .. For assessment of viral immune neutralization in vivo, AAV9-Cre (#105537, Addgene), Bald-LV-Cre, or Mymk+Mymg-LV-Cre were injected into the left TA muscle of (4–5)-week old Mdx 4cv ; Rosa26 tdTomato mice and 28 days later the same viral species were injected into the right TA of the same cohort of mice and muscle were harvested after 28 days and processed for histological analysis. . Immunoblotting Cell, viral, or extracellular vesicle pellets were lysed in NP-40 lysis buffer (50 mM Tris-Cl (pH 8.0), 200 mM NaCl, 50 mM NaF, 1 mM dithiotheritol, 1 mM sodium orthovanadate, 1 mM Benzamidine, 0.3% NP-40 and protease inhibitors). ..
Injection:
Article Title: Corticonigral projections recruit substantia nigra pars lateralis dopaminergic neurons for auditory threat memories
Article Snippet: AAV-DIO-eGFP-Tet-LC (AAVDJ-CMV-DIO-eGFP-2A-TeNT, Stanford University Gene Vector and Virus Core, Cat #GVVC-AAV-71) and AAV-DIO-eGFP (AAVDJ-CMV-DIO-eGFP, Stanford University Gene Vector and Virus Core, Cat #GVVC-AAV-12) were injected bilaterally into TS (ML: ± 3.4, AP: - 0.7, DV: - 3.0) and AuV/TeA (ML: ± 4.5, AP: - 3.0, DV: - 3.4) of C57WT mice. .. AAV9-Cre (AAV0-hSyn-Cre-WPRE-hGH, Addgene Cat # 105553) was injected bilaterally into the SNL (ML: ± 2.0, AP: - 3.0, DV: - 4.1) of C57WT mice. ..
Article Title: Corticonigral projections recruit substantia nigra pars lateralis dopaminergic neurons for auditory threat memories.
Article Snippet: AAV-DIO-eGFP-Tet-LC (AAVDJ-CMV-DIO-eGFP-2A-TeNT, Stanford University Gene Vector and Virus Core, Cat #GVVC-AAV-71) and AAVDIO-eGFP (AAVDJ-CMV-DIO-eGFP, Stanford University Gene Vector and Virus Core, Cat #GVVC-AAV-12) were injected bilaterally into TS (ML: ± 3.4, AP: −0.7, DV: − 3.0) and AuV/TeA (ML: ± 4.5, AP: − 3.0, DV: − 3.4) ofC57WTmice. .. AAV9-Cre (AAV0-hSyn-Cre-WPRE-hGH, Addgene Cat # 105553) was injected bilaterally into the SNL (ML: ± 2.0, AP: − 3.0, DV: − 4.1) of C57WT mice. ..
Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery
Article Snippet: .. For assessment of viral immune neutralization in vivo, AAV9-Cre (#105537, Addgene), Bald-LV-Cre, or Mymk+Mymg-LV-Cre were injected into the left TA muscle of (4–5)-week old Mdx 4cv ; Rosa26 tdTomato mice and 28 days later the same viral species were injected into the right TA of the same cohort of mice and muscle were harvested after 28 days and processed for histological analysis. . Immunoblotting Cell, viral, or extracellular vesicle pellets were lysed in NP-40 lysis buffer (50 mM Tris-Cl (pH 8.0), 200 mM NaCl, 50 mM NaF, 1 mM dithiotheritol, 1 mM sodium orthovanadate, 1 mM Benzamidine, 0.3% NP-40 and protease inhibitors). ..
Article Title: Corticonigral projections recruit substantia nigra pars lateralis dopaminergic neurons for auditory threat memories
Article Snippet: AAV-DIO-eGFP-Tet-LC (AAVDJ-CMV-DIO-eGFP-2A-TeNT, Stanford University Gene Vector and Virus Core, Cat #GVVC-AAV-71) and AAV-DIO-eGFP (AAVDJ-CMV-DIO-eGFP, Stanford University Gene Vector and Virus Core, Cat #GVVC-AAV-12) were injected bilaterally into TS (ML: ± 3.4, AP: − 0.7, DV: − 3.0) and AuV/TeA (ML: ± 4.5, AP: − 3.0, DV: − 3.4) of C57WT mice. .. AAV9-Cre (AAV0-hSyn-Cre-WPRE-hGH, Addgene Cat # 105553) was injected bilaterally into the SNL (ML: ± 2.0, AP: − 3.0, DV: − 4.1) of C57WT mice. ..
Virus:Article Title: Simultaneous analysis of social behaviors and neural responses in mice using round social arena system.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Cre-dependent GCaMP carrying AAV virus: pGP-AAV2/9-Syn-Flex-jGCaMP7f-WPRE Addgene Addgene viral prep #104492-AAV9 Cre-carrying AAV virus: AAV2/9-hSyn-Cre-WRRE-hGH Addgene Addgene viral prep #105553-AAV9 Cre-dependent ChR2-carrying AAV virus: pAAV-hSyn-hChR2(H134R)-EYFP Addgene Addgene viral prep #26973-AAV9 Deposited data Files & codes for building RSA This paper https://doi.org/10.5281/zenodo.6687251 Supplemental figures for this paper This paper Mendeley Data: https://doi.org/10.17632/r5f3jkf2s5.1 Experimental models: Organisms/strains C57BL/6J mouse, 8–10 weeks old, male. .. The Jackson Laboratory Jackson Laboratory Stock #000664 Software and algorithms Ethovision XT Software Suite Noldus https://www.noldus.com/ethovision-xt Arduino Sketch Arduino https://www.arduino.cc/ Inscopix nVista Data Acquisition Software Inscopix https://www.inscopix.com/nvista MATLAB R2020 or above MathWorks https://www.mathworks.com/products/matlab.html (Continued on next page) STAR Protocols 3, 101722, December 16, 2022 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Preform Formlabs https://formlabs.com/software/ Dremel Digilab 3D slicer Dremel https://www.dremel.com/gn/en/digilab/software Other GigE color camera (Basler Ace color camera with Gigabit Ethernet interface) Basler https://www.baslerweb.com/en/products/ cameras/area-scan-cameras/ace/ SVPRO Fisheye Lens 180 Degree USB Camera Module SVPRO https://www.amazon.com/SVPROIllumination-Camera-ModuleFisheye/dp/B07C1N624T Inscopix nVista system (DAQ + microendoscopic camera) Inscopix https://www.inscopix.com/nvista Proview GRIN lens (1 mm diameter, 4 mm length, 0.5 NA) Inscopix 1050-004605 Prizmatix optogenetics LED-Dual Prizmatix https://www.prizmatix.com/Optogenetics/ Optogentics-LED-Dual.aspx#Header Prizmatix USB pulser Prizmatix https://www.prizmatix.com/Optogenetics/PulserPlus.htm Fiber optic cannulae with 1.25 mm diameter ceramic ferrule, 400 mm core, 0.5 NA RWD Life Science Inc. R-FOC-L400C-50NA Mini USB I/O Box Noldus https://www.noldus.com/ethovision-xt/io-box Arduino Uno Arduino https://www.arduino.cc/ Form2 Formlabs https://formlabs.com/ 3D20 Dremel https://www.dremel.com/gn/en/p/ dremel-3d20-idea-builder-3d20-01-v680 Formlabs Clear Resion v4 Formlabs RS-F2-GPCL-04 PLA-White Filament, 1.75 mm Diameter Dremel PLA-WHI-01 PC817 opto-coupler Gikfun N/A Resistors (220 ohm, 10k ohm) BOJACK N/A ll OPEN ACCESS Protocol
Bioprocessing:Article Title: Simultaneous analysis of social behaviors and neural responses in mice using round social arena system.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Cre-dependent GCaMP carrying AAV virus: pGP-AAV2/9-Syn-Flex-jGCaMP7f-WPRE Addgene Addgene viral prep #104492-AAV9 Cre-carrying AAV virus: AAV2/9-hSyn-Cre-WRRE-hGH Addgene Addgene viral prep #105553-AAV9 Cre-dependent ChR2-carrying AAV virus: pAAV-hSyn-hChR2(H134R)-EYFP Addgene Addgene viral prep #26973-AAV9 Deposited data Files & codes for building RSA This paper https://doi.org/10.5281/zenodo.6687251 Supplemental figures for this paper This paper Mendeley Data: https://doi.org/10.17632/r5f3jkf2s5.1 Experimental models: Organisms/strains C57BL/6J mouse, 8–10 weeks old, male. .. The Jackson Laboratory Jackson Laboratory Stock #000664 Software and algorithms Ethovision XT Software Suite Noldus https://www.noldus.com/ethovision-xt Arduino Sketch Arduino https://www.arduino.cc/ Inscopix nVista Data Acquisition Software Inscopix https://www.inscopix.com/nvista MATLAB R2020 or above MathWorks https://www.mathworks.com/products/matlab.html (Continued on next page) STAR Protocols 3, 101722, December 16, 2022 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Preform Formlabs https://formlabs.com/software/ Dremel Digilab 3D slicer Dremel https://www.dremel.com/gn/en/digilab/software Other GigE color camera (Basler Ace color camera with Gigabit Ethernet interface) Basler https://www.baslerweb.com/en/products/ cameras/area-scan-cameras/ace/ SVPRO Fisheye Lens 180 Degree USB Camera Module SVPRO https://www.amazon.com/SVPROIllumination-Camera-ModuleFisheye/dp/B07C1N624T Inscopix nVista system (DAQ + microendoscopic camera) Inscopix https://www.inscopix.com/nvista Proview GRIN lens (1 mm diameter, 4 mm length, 0.5 NA) Inscopix 1050-004605 Prizmatix optogenetics LED-Dual Prizmatix https://www.prizmatix.com/Optogenetics/ Optogentics-LED-Dual.aspx#Header Prizmatix USB pulser Prizmatix https://www.prizmatix.com/Optogenetics/PulserPlus.htm Fiber optic cannulae with 1.25 mm diameter ceramic ferrule, 400 mm core, 0.5 NA RWD Life Science Inc. R-FOC-L400C-50NA Mini USB I/O Box Noldus https://www.noldus.com/ethovision-xt/io-box Arduino Uno Arduino https://www.arduino.cc/ Form2 Formlabs https://formlabs.com/ 3D20 Dremel https://www.dremel.com/gn/en/p/ dremel-3d20-idea-builder-3d20-01-v680 Formlabs Clear Resion v4 Formlabs RS-F2-GPCL-04 PLA-White Filament, 1.75 mm Diameter Dremel PLA-WHI-01 PC817 opto-coupler Gikfun N/A Resistors (220 ohm, 10k ohm) BOJACK N/A ll OPEN ACCESS Protocol
Western Blot:Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery
Article Snippet: .. For assessment of viral immune neutralization in vivo, AAV9-Cre (#105537, Addgene), Bald-LV-Cre, or Mymk+Mymg-LV-Cre were injected into the left TA muscle of (4–5)-week old Mdx 4cv ; Rosa26 tdTomato mice and 28 days later the same viral species were injected into the right TA of the same cohort of mice and muscle were harvested after 28 days and processed for histological analysis. . Immunoblotting Cell, viral, or extracellular vesicle pellets were lysed in NP-40 lysis buffer (50 mM Tris-Cl (pH 8.0), 200 mM NaCl, 50 mM NaF, 1 mM dithiotheritol, 1 mM sodium orthovanadate, 1 mM Benzamidine, 0.3% NP-40 and protease inhibitors). ..
Lysis:Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery
Article Snippet: .. For assessment of viral immune neutralization in vivo, AAV9-Cre (#105537, Addgene), Bald-LV-Cre, or Mymk+Mymg-LV-Cre were injected into the left TA muscle of (4–5)-week old Mdx 4cv ; Rosa26 tdTomato mice and 28 days later the same viral species were injected into the right TA of the same cohort of mice and muscle were harvested after 28 days and processed for histological analysis. . Immunoblotting Cell, viral, or extracellular vesicle pellets were lysed in NP-40 lysis buffer (50 mM Tris-Cl (pH 8.0), 200 mM NaCl, 50 mM NaF, 1 mM dithiotheritol, 1 mM sodium orthovanadate, 1 mM Benzamidine, 0.3% NP-40 and protease inhibitors). ..
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